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KMID : 0545120180280040613
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Journal of Microbiology and Biotechnology
2018 Volume.28 No. 4 p.613 ~ p.621
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Preparation of Interface-Assembled Carbonyl Reductase and Its Application in the Synthesis of S-Licarbazepine in Toluene/Tris-HCl Buffer Biphasic System
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Ou Zhimin
Xu Jiahui
Du Lihua
Tang Lan
Niu Yangping
Cui Jian
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Abstract
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In this study, interface-assembled carbonyl reductase (IACR) was prepared and used in the synthesis of S-licarbazepine in a toluene/Tris-HCl biphasic system. The carbonyl reductase (CR) was conjugated with polystyrene to form a surfactant-like structure at the interface of the toluene/Tris-HCl biphasic system. The interface-assembled efficiency of IACR reached 83% when the CR (180 U/mg) and polystyrene concentration were 8 × 102 g/ml and 3.75 × 103 g/ml, respectively. The conversion reached 95.6% and the enantiometric excess of S-licarbazepine was 98.6% when 3.97 × 106 nmol/l oxcarbazepine was converted by IACR using 6% ethanol as a co-substrate in toluene/Tris-HCl (12.5:10) at 30°C and 43 ×g for 6 h. IACR could be reused efficiently five times.
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KEYWORD
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Asymmetric reduction, interface-assembled carbonyl reductase, oxcarbazepine, polystyrene, S-licarbazepine
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